Synthesis of an array comprising 551 variants of the FBP28-WW protein domain

摘要

WW domains are protein-protein interaction modules composed of 35-50 amino acids.They are the smallest naturally occurring,monomeric,triple-stranded,anti-parallel beta-sheet protein domains.The WW domains bind proline-rich or proline-containing ligands with dissociation constants from of 1 up to 50 mu M.The FBP28 WW-domain(formin binding protein)is quite stable(Mp = 61 deg C),monomeric and monodisperse in solution.We use it as a model system for binding studies.