Proliferation of human osteoblastic cells and synthesis of extracellular bone matrix on biomaterials

摘要

Primary human osteoblasts were cultered on biomaterials to develop living bone substitutes by Tissue Engineering. Monolayer cultures were established from iliac crest biopsies. Single cell suspensions were then seeded onto tricalciumphosphate (TCP) BIOBASEcircle R, hydroxyapatite-collagen(HAC), autoclaved cancellous bone (CB) and fibrin glue (FG) TissucolcircleR. The constructs were analyzed by electronmicroscopy, Cell proliferation assay (XTT) and histology to assess adhesion, proliferation and extracellular matrix formation on the biomaterial. The osteoblastic cells survived in the fibrin glue and established a mesenchymal phenotype. They adhered to the HAC-sponges, TCP and CB. Prolifertion was seen on TCP and CB. HAC had a suppressive effect on the cells. An inmature matrix was found on TCP and a bone neomatrix on CB after 4 weeks in vitro.