Inhibitory Activity of Tyrosinase Enzyme on Lotion Contains Pear (Pyrus pyrifolia (Burm.F) Nakai) Rind Extract

摘要

Objectives: The purpose of this study was to make lotion preparations containing ethanol extract of pear rind and to test the inhibitory activity of the tyrosinase enzyme from pear rind ethanol extract lotion preparations. Method: Determination of tyrosinase enzyme inhibitory activity was carried out using a microplate reader instrument. The formula used is ethanol extract of pear rind, stearic acid, cetyl alcohol, triethanolamine, liquid paraffin, glycerin, sodium benzoate, essense pear and distilled water. Results and Discussion: Ethanol extract of pear rind has tyrosinase enzyme inhibiting activity which is indicated by IC_(50) values of 520.45 μg/mL, and in lotion preparations it has tyrosinase enzyme inhibitory activity which is indicated by IC_(50) values of 599.96 μg/mL. Conclusion: From the results of tyrosinase inhibitory activity test, lotion containing ethanol extract of pear rind has the potential to be a skin lightening.