Monitoring Constituent Concentrations in Bioreactions With Attenuated Total Reflection Fourier Transform Infrared Spectroscopy

摘要

Reaction mixtures of enzyme catalyzed hydrolysis of penicillin and sugar concentrations in plant cell culture were monitored with attenuated total reflection Fourier transform infrared spectroscopy(ATR/FT-IR). Partial least square algorithm was used for estimation and measurement errors for penicillin, 6-aminopenicillanic Acid (6-APA), phenylacetic acid(PAA), glucose, fructose, and sucrose were 0.45, 0.53, 0.59, 0.35, 0.22, and 0.11 g/L respectively in the solutions fro calibration. Concentration profiles for these compounds were obtained by using ATR/FT-IR in real reaction and cultivation processes.