Spectroscopic Characteristics and Energy Transfer of Bacterial Pigment: (Pyocyanin / Curcumin)

摘要

In this research used a new strategy in the detection of residues Pseudomonas aeruginosa (Pyocyanin), using the fluorescence spectrum alongside the absorption spectrum of the detection of metabolite in pure water. The bacterial metabolite was successfully measured, by taking the clinically relevant range into account (7-130 μM); at a concentration of [10 μm]. Were measured, the Pyocyanin solutions spectrum consist of three peaks and one shoulder, located at wavelengths (230 nm), (316nm), (370nm), (700 nm), respectively, but the concentration [1μM], its absorption spectrum doesn't appear clearly and disappears due to dilution, spectra of fluorescence have been measured As in the case of absorption, at optimal wavelength excitation (380 nm), band have appeared at wavelength (443 nm) at all concentrations. To calculate energy transfer, it must be calculated Forster's radius Ro for FRET between Pyocyanin molecule as a donor was calculated with the Curcumin molecule as an acceptor and its value was Ro=7.6235 ?A, this is the limit lower for Forester's energy transfer from molar absorptivity of concentration [100 μm] from spectral overlap by used the MatLab program. Then, calculated energy transfer values and the quenching factor, When mixing the Curcumin dissolved in water with Pyocyanin were studied at different concentrations at room temperature at ratio (1/99), (7/93), (11/89). It was found that the increase of energy transfer from the donor to the acceptor with the increasing of the donor maxing rate, the quenching factor is reduced by increasing the presence of the donor and values do depend on concentration. We have achieved great importance in this research by knowing the spectral properties of the respective bacterial dyes. Thus, the data obtained is useful enough to be relied upon as a database for Pyocyanin dye spectra.