Effects of Ti, Ti_6Al_4V, and Ti_6Al_7Nb on Osteoblast Bioactivity

摘要

The aim of this study was to investigate the surface morphology, hydrophilicity, and effects of sandblasted and acid-etched Ti, Ti_6Al_4V, and Ti_6Al_7Nb on the bioactivity of osteoblasts. The surfaces of Ti, Ti_6Al_4V, and Ti_6Al_7Nb were treated with Al_2O_3 sandblasting and a hydrochloric acid-sulfuric acid mixture to create etching. Surface morphology of samples was observed using scanning electron microscopy. The size of the contact angle was measured under a microscope using a measuring instrument. Osteoblasts from Sprague-Dawley rats were inoculated onto the surfaces of Ti, Ti_6Al_4V, and Ti_6Al_7Nb at a density of 1×10~4 cells/ml. Cell morphology was observed using scanning electron microscopy. After sandblasting and acid etching, the surfaces of Ti, Ti_6Al_4V, and Ti_6Al_7Nb presented micron-size porous morphology and were hydrophilic. Cells spread significantly on the sandblasted and acid-etched surfaces of all three surfaces. Adhesion of osteoblasts was strongest on the surface of Ti_6Al_7Nb. Sandblasting and acid etching promoted bioactivity on all three surfaces, although sandblasted and acid-etched Ti_6Al_7Nb showed better bioactivity than Ti or Ti_6Al_4V. Overall, osteoblasts on the surface of Ti6Al7Nb exhibited better adhesion.