METHOD FOR GROWING OSTEOBLAST CELL BASED ON TI/TIO2 NANOTUBE

摘要

The present invention relates to a method for growing an osteoblast cell based on a Ti/TiO2 nanotube. More particularly, by culturing the osteoblast cell after irradiating an electron beam on the Ti/TiO2 nanotube manufactured by an anodic oxidation method, an adsorption ratio can be increased more than that of before the electron beam irradiation, and by improving or controlling material properties by irradiating an electron beam on the Ti/TiO2 nanotube, not only properties as new functional material can be provided but also high added values can be created. Thereby, material wherein the osteoblast cell based on the Ti/TiO2 nanotube are generated can be applied as materials for dental implant, restoring treatment of bone defect, recycling an alveolar bone, bone graft, bone augmentation or auxiliary materials.;COPYRIGHT KIPO 2014;[Reference numerals] (a) Step of cutting Ti foil and Pt foil into a fixed size; (b) Step of putting the cut Ti foil and Pt foil into an beaker containing ethanol, cleaning it using an ultrasonic cleaner for 3 minutes and drying it using nitrogen gas; (c) Step of manufacturing 1000 ml of a 0.5% HF aqueous solution by mixing 10.4 ml of a 48% HF solution and 989.6 ml of DI water; (d) Step of approving external voltage of 15-25 V after connecting the Pt foil to a cathode of power supply and Ti foil to an anode thereof and putting the two foils into the 0.5% HF aqueous solution to become parallel for the purpose of using an anodic oxidation method; (e) Step of taking out the Ti foil from the HF solution and sufficiently cleaning it and then drying it using nitrogen gas; (f) Step of drying the dried Ti foil for 3-5 hours at a temperature of 80°C by putting it in an electric oven; (g) Step of manufacturing a Ti/TiO2 nanotube by putting the completely dried Ti foil into an electric furnace in air and then performing a heat treatment for 1 hour a temperature of 100°C; (h) Step of irradiating an electron beam for enhancing osteogenesis and Synostosis on the Ti/TiO2 nanotube; (i) Step of culturing preosteoblast cells (MC3T3-E1) using a DMEM medium wherein a 10% fetal bovine serum (FBS) is added after irradiating the electron beam, and culturing it on the Ti/TiO2 nanotube for 2-24 hours