Effect of Polysaccharides from Fresh Persimmon (Diospyros kaki) Fruits and its Sulfated Derivates on the Immunomodulatory Activity of Mouse Peritoneal Macrophage Cells

摘要

The effects of polysaccharides extracted from persimmon (Diospyros kaki) fruits (PFP) and its sulfated derivates (PFP-S) on activity of mouse peritoneal macrophages (PM) were evaluated by measuring their effects on the viability, nitric oxide (NO) production, cytokine (TNF-α, IL-10 and IL-2) production and gene expression. The results showed that both PFP and PFP-S significantly increased the viability and the productions of NO, TNF-α and IL-10 with the increase of the substitution degree (DS) and dose. Of the fractions, PFP-SIII (DS 2.5, Mw 48 kDa) was the most potent inducer of NO and cytokine production, increasing production of NO and expression of cytokines (IL-10 and TNF-α). It was supposed from this result that PFP-S might act as an inducer of macrophage functions against pathogens, which might be affected by the sulfate content of polysaccharides made great role in regulate of immunomodulatory activities.