Nucleic Acids Chemistry beyond the Watson-Crick Double Helix(58):The effect of molecular crowding on the stability of RNAG-quadruplexes with various number of quartets and loops

摘要

RNA G-quadruplexes are present across the transcriptome in various regions ofmRNAs and non-coding RNAs,and play important roles in key cellular functions.Intracellular environment is highly crowded due to the presence of high concentrations ofbiomolecules,leading to molecular crowding conditions.It has been demonstrated that thestabilities of G-quadruplexes are significantly sensitive to the molecular crowding.Tounderstand and regulate functions controlled by RNA G-quadruplexes,it is important topredict the stability of folded complexes that are adopted by sequences with differentnumber of G-quartets and variable-length loops between them under the molecularcrowding conditions.