In Vitro Reproduction and Ex Vitro Adaptation of Complex Resistant Grape Varieties

摘要

The research object is complex-resistant grape varieties. Intensively growing green shoots of grapes were taken as initial samples which were cut into single-eye cuttings and then meristems were extracted in laminar boxes. Experiments have shown that regeneration of shoots from extracted apexes occurred at all 6-BAP concentrations. 6-BAP has a positive effect at concentrations of 0,5-1,0 mg/l. To accelerate elongation of microshoots, we studied effects of the gibberellic acid combined with 6-BAP at various concentrations. The combination of 0,5 mg/l 6-BAP + 1,0 mg/l HK showed the best results. This combination accelerated the growth of plant stems, and two weeks after, the shoot was 25-26 mm in size. At the ex-vitro plant adaptation stage, positive lignohumate effects were studied. The ability to increase plant resistance to adverse environmental factors was of special interest. The roots were put into an aqueous solution., The substrate was also watered with this solution immediately after replanting. In order to establish the optimal effect, various drug concentrations were studied. Distilled water was used as a control factor. All concentrations of lignohumate had a positive effect on plants. It is necessary to note that lignohumate increased the leaf area. At concentration of 1,0 g/l, the height of plants ha increased by the 30th day after adaptation.