Preparation of New Acellular Dermal Collagen Scaffolds via Combination of Phytoproteinases and Supercritical Carbon Dioxide Treatments

摘要

Tissue-engineering technology was important and popular which combine medical applications and engineering materials knowledge such as a cell extraction process, which was employed to remove the cellular components from porcine dermal, leaving a matrix of collagen, elastin, collagen, and tightly bound glycosaminoglycans (GAGs). Said cell extraction process was used to remove cellular components from porcine dermal materials. Different porosities and microstructure of the acellular tissues were could be created by using different concentrations of related enzymes and collagenase. In this study, the uniform porcine dermal would be obtained by using a designed machine. It get dermis exactly and quickly via a machine which was designed from our team (powerdriven dermatome, SIACIN international, SC-GST). The porcine dermal must remove fat and other impurities almost by supercritical fluid (SCF), otherwise adipose tissue will become rot immediately. For collagen scaffold samples, dermis were hydrolyzed by enzymes of different concentration, in this way it can gain stant completely and clearly. Electron microscopy revealed effect in extracellular matrix (ECM) of porcine dermal tissue via supercritical fluid and different concentrations enzymes. This suggests the enzymes concentration and when you put porcine dermal in SCF was mightily important for effect of the collagen.