Optical sensing platform for colorimetric determination of silver nanoprisms and its application in hydrogen peroxide and glucose detections using a mobile device camera

摘要

Silver nanoprisms (AgNPrs) have a unique localized surface plasmon resonance, resulting in strong absorption and scattering within the visible light region. In this work, we propose the image acquisition from colloidal solutions of AgNPrs using a combination of transmitted and scattering lights. The developed measurement technique could be carried out by separately recording transmitted and scattering images of the solutions, using a mobile device camera prior to calculation of empirical absorption value (IA). The instrumental setup is shown in Fig. 1A. The IA value of green for AgNPrs solutions was found in agreement with the absorption spectra obtained using a conventional spectroscopic technique. This technique was utilized for the quantifications of hydrogen peroxide (H2O2) and glucose. The transmitted and scattering images of AgNPrs solutions in the present of a series of glucose solutions with concentrating ranging from 20 to 100 ?M are shown in Fig. 1B. The good linearities between ?IA and those typical analytes were observed (Fig. 1B). The limit of detection for typical biosensor of glucose was 19.8 ?M. We expect the methodology herein developed for H2O2 and glucose determinations by means of monitoring the color change of transmitted and scatting images from solutions, to contribute to the development of simple, rapid, and reliable detection systems to be further applied in biochemical analysis and clinical diagnosis, as well as in household biosensor applications.