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Optical Sensing Platform for the Colorimetric Determination of Silver Nanoprisms and Its Application for Hydrogen Peroxide and Glucose Detections Using a Mobile Device Camera

机译:比色法测定银纳米棱镜的光学传感平台及其在移动相机中过氧化氢和葡萄糖检测中的应用

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Silver nanoprisms (AgNPrs) have a unique localized surface plasmon resonance, resulting in strong absorption and scattering within the visible light region. In this work, we propose image acquisition from colloidal solutions of AgNPrs using a combination of transmitted and scattered light. The developed measurement technique could be carried out by separately recording transmitted and scattering images of the solutions, using a mobile device camera prior to a calculation of the empirical absorption value (I _(A)). The I _(A) value of green for AgNPrs solutions was found to be in agreement with the absorption spectra obtained using a conventional spectroscopic technique. This technique was utilized for the quantifications of hydrogen peroxide and glucose. Good linearities between ΔI _(A) and those typical analytes were observed. The limit of detection for the typical biosensor of glucose was 19.8 μM. As such, we expect the methodology herein developed for hydrogen peroxide and glucose determinations by means of monitoring the color change of transmitted and scatting images from solutions to contribute to the development of simple, rapid, and reliable detection systems to be further applied to biochemical analysis and clinical diagnosis, as well as to household biosensor applications.
机译:银纳米棱镜(AgNPrs)具有独特的局部表面等离子体激元共振,导致在可见光区域内强烈吸收和散射。在这项工作中,我们建议使用透射光和散射光的组合从AgNPrs的胶体溶液中获取图像。可以通过在计算经验吸收值(I_(A))之前使用移动设备摄像机分别记录溶液的透射图像和散射图像来执行开发的测量技术。发现AgNPrs溶液的绿色的I_(A)值与使用常规光谱技术获得的吸收光谱一致。该技术用于过氧化氢和葡萄糖的定量。观察到ΔI_(A)与那些典型分析物之间的良好线性。典型的葡萄糖生物传感器的检出限为19.8μM。因此,我们希望通过监测溶液中透射图像和散斑图像的颜色变化,在此开发用于过氧化氢和葡萄糖测定的方法,有助于开发简单,快速,可靠的检测系统,从而进一步应用于生化分析和临床诊断,以及家用生物传感器的应用。

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