Proliferation Activity of Mucosal Epithelial Cell Nucleus With AgNOR Staining in Smokers and Non- Smokers

摘要

Oral cancer detection is difficult in the early stages leading to high mortality rate of the disease. Therefore, a new method of early detection of oral carcinoma is required as treatment in the early stages is more effective at minimizing morbidity. The purpose of this study was to examine the differences in cellular structure of AgNOR nuclear proliferation activity collected from normal buccal mucosa with cytology exfoliative techniques in smokers and non-smokers. Nucleolar Organizer Region (NOR) is a DNA that encodes RNA ribosomes and is considered essential in protein synthesis. Calculation of AgNOR is considered as an index to estimate the potential of cellular mitosis. This is a descriptive analytic research with cross sectional approach. Data were collected at the Oral Medicine Installation, RSGMP FKG USU and laboratory examination was performed at the Anatomical Pathology Laboratory, Faculty of Medicine USU in January and February 2014. The number of research subjects was 20 people. The data were obtained by questionnaires and clinical examination on the subjects who met the inclusion criteria. Data analysis is done by testing the hypothesis done on univariate data and bivariate data. The collected data were analyzed with Mann-Whitney statistical test and unpaired t-test. The results of this study indicated that there were significant differences to the count of AgNOR smokers and non-smokers. The proliferation activity of buccal mucosal epithelial cells using AgNOR dye smokers and non-smokers showed significant difference (P = 0.0001). There is no significant relationship between the duration, type and number of cigarettes smoked per day. But the differences can be seen pathologically. In conclusion, there is a significant association between smoking and an increase in cell proliferation activity of the oral mucosa in smokers.