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Method for the ex vivo cultivation of oral mucosal epithelial progenitor cells and oral mucosal epithelial cells
Method for the ex vivo cultivation of oral mucosal epithelial progenitor cells and oral mucosal epithelial cells
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机译:口腔粘膜上皮祖细胞和口腔粘膜上皮细胞的离体培养方法
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摘要
A method for the ex vivo cultivation of oral mucosal epithelial progenitor cells and oral mucosal epithelial cells includes: subjecting an oral mucosal tissue to an enzymatic digestion treatment with collagenase, so as to obtain cell aggregates which include oral mucosal epithelial progenitor cells and oral mucosal epithelial cells; cultivating the cell aggregates with an amniotic membrane in a serum-free platelet lysate-containing medium in the absence of feeder cells, so that the cell aggregates are adhered onto the amniotic membrane; and subsequently cultivating the cell aggregates adhered on the amniotic membrane in a serum-free proliferation-facilitating medium in the absence of feeder cells.
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