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Cross-linked fibrotic collagen gel for oral mucosal epithelial cell culture
Cross-linked fibrotic collagen gel for oral mucosal epithelial cell culture
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摘要
PROBLEM TO BE SOLVED: To provide a cell culture substrate whose constituent is collagen and whose contraction is suppressed during the culture of oral mucosal epithelial cells including gas-liquid interface culture. SOLUTION: This cell culture substrate is composed of (i) intact crosslinked fibrotic collagen, and (ii) a region containing concave and/or convex shapes on at least a part of its surface. Having (iii) uncrosslinked fibrotic collagen gel, after being chemically crosslinked by a carbodiimide-based crosslinking agent, crosslinked by γ-ray irradiation in the presence of an aqueous solvent, (i) ~ (iii It is a cross-linked fibrotic collagen gel that satisfies all the conditions of 1). [Selection diagram] Fig. 3
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