Bioinformatics Analysis of the 1-Deoxy-D-Xylulose-5-Phosphate Synthase (DXS) Gene in Cabbage (Brassica Oleracea Var. Capitata)

摘要

1-deoxy-D-xylulose-5-phosphate synthase (DXS) is an rate-determing enzyme in carotenoid biosynthesis. Here, the Brassica oleracea var. capitata DXS (BocDXS) gene sequences were obtained from Brassica database (BRAD), and preformed for bioinformatics analysis. The BocDXS1, BocDXS2 and BocDXS3 genes mapped to chromosomes 3, 7 and 9, and contains an open reading frame of 1,893 bp, 2,049 bp and 2,088 bp that encodes a 712, 682, 695 amino acid protein, respectively. Subcellular localization predicted all BocDXS genes were in the chloroplast. The conserved domain of the BocDXS protein is PLN02582. Homology analysis indicates that the DXS protein is apparently conserved during plant evolution. The findings of the present study provide a molecular basis for the elucidation of DXS gene function in cabbage.