Biological effect of the 50 Hz Magnetic Field in a three-dimension (3D) in vitro experimental model of SH-SY5Y human neuroblastoma cells

摘要

Given the reported limitations of the conventional 2D in vitro cultures of neuronal cells in terms of response to drugs and radiations, we here aimed at evaluating the effects elicited by the 50-Hz magnetic field (MF) on the human neuroblastoma SH-SY5Y cells grown in a new three-dimension (3D) scaffold in vitro. We demonstrate that (i) no change in proliferation, distribution of cell cycle phases and viability, and no induction of apoptosis is triggered by the MF in the SH-SY5Y cells if cultured in the 3D scaffolds; (ii) the intracellular GSH content, as well as the expression of both the gamma-GCS (responsible for the GSH biosynthesis) and SOD1 genes are impaired by the MF exclusively in cells grown in the 3D culture conditions.