Study of 3-cyanovinylcarbazole mediated DNA photo-cross-linking by using uracil derivatives

摘要

DNA photo-cross-linking is a useful tool for the regulation, manipulation, and detection of nucleic acids. We have already reported that the oligonucleotide containing 3- cyanovinylcarbazole(~(CNV)K) can crosslink to the pyrimidine base in the complementary strand with a few seconds of photoirradiation1. In this study, we investigated the effect of 5- substitution of the pyrimidine base in DNA photo-cross-inking using ~(CNV)K.