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THERMOLABILE URACIL DNA GLYCOSYLASE, METHOD FOR THE PRODUCTION THEREOF AND USE FOR REMOVING URACIL FROM DNA
THERMOLABILE URACIL DNA GLYCOSYLASE, METHOD FOR THE PRODUCTION THEREOF AND USE FOR REMOVING URACIL FROM DNA
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机译:尿嘧啶尿嘧啶DNA糖基化酶,其生产方法和用于从DNA中去除尿嘧啶的方法
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摘要
Thermolabile enzyme with uracil-DNA-glycosylase activity which is in particular characterized by a high degree of purity, short half-lives and a content of contaminating foreign activities of less than 2%, a process for its isolation as well as the use thereof to remove the base uracil from DNA and in particular from PCR products containing uracil. The enzyme is obtainable from gram-positive microorganisms such as e.g. Arthrobacter or Micrococcus.
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