Microfluidics-based single-molecule live-cell imaging on cellular interactions occurring in the presence of external force

摘要

Visualizing and characterizing nanoscopic spatiotemporal dynamics of cellular interactions occurring in the presence of external force at the molecule level has been challenging although they play a key role in my biologically important processes. Using an initial step of hematopoietic stem/progenitor cell (HSPC) homing as a model system, we develop a new fluorescence imaging platform that enables to capture and quantify spatiotemporal behavior of ligand-receptor interactions that control the initial step of the HSPC homing.