A new method for determination of herbicide resistance: using Diversity Array Technology to determine annual ryegrass resistance to trifluralin(Group D herbicide)

摘要

Annual ryegrass (Lo/ium rigidum Gaud.) is considered the worst winter crop weed of Australia. This is due in part to its propensity to evolve resistance to herbicides, having evolved resistance to 11 different herbicide groups. As levels of herbicideresistance in Australia are increasing, new methods are needed to assist in the early identification and treatment of resistant weeds. Conventional methods for the determination of resistance involve glasshouse pot trials, Petri dish assays or enzymaticdetermination, all of which consume considerable time and resources. A pilot study was conducted to determine the feasibility of utilising diversity arrays technology (DArT) as a faster and cheaper method for herbicide resistance testing. DArT is a highthroughput genotyping technology which utilises microarray s for the discovery of genetic markers. The aim was to identify genetic markers in resistant and susceptible biotypes of annual ryegrass and to assess the ability of DArT to discriminate betweenherbicide resistant phenotypes.