Fabrication Three-Dimensional Microporous Nanofibrous Bacterial Cellulose Scaffold by Gelatin Template

摘要

Cells are inherently sensitive to their three-dimensional (3D) environments from the macroscale, microscale, to the nanoscale. The nanoscale structure of the extracellular matrix (ECM) provides a natural network of nanofibers (30~100 nm) to support cells and guide their behavior. Conventionally, biomaterial scaffolds for tissue regeneration have been designed macroscopically to match the properties of the organs they are to replace~1. Microporous scaffolds with the appropriate pore sizes (100-400 μm) and porosities (-90 %) have been fabricated to support cell migration, proliferation, vascularization and tissue ingrowth~2. Moreover, it has been proved the nanofibrous scaffold can promote cell attachment, proliferation and expression of matrix components~3. Therefore, it is necessary to develop a facile method to fabricate tissue regeneration biomaterial with a 3D microporous nanofibrous scaffold structure for more biomimetic cellular environment.