SERS-active metal-dielectric nanostructures integrated in microfluidic devices for ultra-sensitive label-free miRNA detection

摘要

In this work, silver decorated porous silicon membranes integrated in a polydimethylsiloxane multi-chamber microfluidic chip were functionalized with DNA-probes and used for the detection of miRNA by Surface-enhanced Raman Scattering analysis. An innovative biological protocol has been designed;;the probe was divided in two short pieces that interact before and after the miRNA incubation. The optofluidic biosensor was applied for the label-free detection of miRNA sequences at in vivo concentrations.