The effect of sampling strategy on porcine epidemic diarrhea virus (PEDV) associated gut morphology and immunohistochemistry scores

摘要

Porcine epidemic diarrhea virus (PEDV) lesions are confined to the small intestine and consist of villous atrophy and fusion. Histological examination, immunohistochemistry (IHC), and vil-lous-to-crypt height ratio (VCR) indicate the presence of PEDVand provide estimates of the disease severity, but rely heavily on high quality fixed tissues and the selection of affected gut regions for sampling. The objective of this trial was to compare lesions, IHC scores and VCRs between PEDV positive and negative pigs and between two different intestinal sampling strategies, the W and L methods, to generate recommendations on intestinal sampling strategies to optimize diagnostic testing results.