MICRORNAS MIR-21 AND MIR-181C IN CEREBROSPINAL FLUID AND SERUM IN CANINE MENINGOENCEPHALOMYELITIS OF UNKNOWN ORIGIN

摘要

The purpose of this study was to determine and compare the expression of microRNAs miR-21 and miR-181c in cerebrospinal fluid (CSF) and serum of dogs with and without meningoencephalomyelitis of unknown origin (MUO).CSF and serum samples were obtained from ten dogs with MUO (MUO group) and from eight dogs with non-inflammatory neurological diseases with a normal CSF nucleated cell count (NCC) that were selected as control group (NINDC). Serum from four healthy dogswas used as second control group (HC). Relative expression of miR-21 and miR-181c was determined by qRT-PCR using the AACt method after adding cel-miR-39 as a spike-in control. Results were compared between groups using a general linear model. Pearson correlation analysis assessed association between parameters.