Accurate Quantification and Quality Control of Illumina TruSeq Sequencing Libraries with the QX100? Droplet Digital? PCR (ddPCR?) System

摘要

A ddPCR method has been developed to accurately perform quantification and quality control (QC) of Illumina TruSeq libraries. A FAM/HEX duplex assay designed to Illumina's TruSeq adapters allows for the detection of species comprised of the P5 and P7 adapters. ddPCR quantification can exclude ill-formed libraries and adapter dimers, which would not yield valuable sequence information on the insert. The accurate quantification allows a researcher to load an optimal amount of library within the narrow optimal range, thereby maximizing the amount of useable sequencing reads without saturating the system.