AMINATED ELASTIN-LIKE POLYPEPTIDE COPOLYMER COATINGS FOR LIVER CELL CULTURE

摘要

We have developed a method using the OPA flourescence assay to facilitate the creation of ELP copolymer coatings with varying levels of amination. Our results suggested that both the type of the charge group (PEI or PL) and the level of surface charge affected hepatocyte differentiation. This result provides a starting point for extensive mechanistic studies in how charge type and content affect long term cell culture performance.