Evaluation of the Promega Maxwell? CSC and the Maxwell? CSC DNA FFPE Kit

摘要

Introduction: Personalized medicine for oncology patients is increasingly based upon mutation detection in cancerous tissues, typically stored as Formalin Fixed Parafin Embedded (FFPE) tissues. An important component of the workflow needed for biomarker discovery is a DNA extraction method that produces high quality DNA, suitable for high resolution downstream analysis, including next generation sequencing. FFPE samples are difficult samples for molecular labs as the fixation process causes DNA fragmentation and protein-DNA cross linking. Frequently the tissues also contain inhibitors and/or quenching substances that must be removed in the extraction process. In this research study, we provide a side-by-side evaluation of the Promega Maxwell CSC DNA FFPE automated extraction and the Roche Life Sciences MagNaPure method for isolating DNA from FFPE tissues of various tumor types.