A Conjugation Approach to Produce a S. aureus Synbody with Bactericidal Activity in Serum

摘要

Maleimide conjugation is a widely used conjugation strategy due to its high yield, selectivity, and low cost. Here we use this strategy to conjugate two antibacterial peptides to produce a bivalent antibacterial peptide, called a synbody that has bactericidal activity against methicillin resistant Staphylococcus aureus (MRSA). The synbody was prepared by conjugation of a partially D-amino acid substituted synthetic antibacterial peptide to a bis-maleimide scaffold.