Utilizing Chemically Self-Assembled Antibody Nanorings (CSANs) as Bispecific Targeting Agents for Cell-Directed Immunotherapy

摘要

Chemically Self-Assembled Nanorings (CSANs) are formed by engineering fusion proteins that contain a single-chain variable fragment (scFv) fused to two E. Coli dihydrofolate reductase (DHFR2) molecules which spontaneously self-assemble into octameric nanorings following the addition of a chemical dimerizer, bis-methotrexate (BisMTX). Our laboratory has designed a non-genetic system in which two different scFv-displaying fusion proteins can be combined into a single nanoring, thereby targeting multiple cell surface biomarkers simultaneously.