The Study on Poly-lysine-alginate Microcapsules Mediated Virus Genomic DNA Transfection

摘要

The goal in this paper is to investigate the efficiency of poly-PLL (Poly-L-lysine)/Alg (Alginate) vector mediated virus genomic DNA transfection and the virus genomic DNA's biological activity in vivo. After Pseudorabies virus (PRV) genomic DNA being adhered to the porous CaCO_3 particles, PLL and Alg were alternately polymerized on the surface of the porous DNA-CaCO_3 particles to 7 layers, which were later dissolved them by EDTA to remove CaCO_3 cores; the vectors in which the DNA were coated by poly-PLL/Alg, were harvested to infect the rabbits and observe the replication of viral DNA. Porous CaCO_3 particles, which were obtained from the reaction between Na_2CO_3 and CaCl2, had an efficiency of absorbing DNA 1 μg/mg CaCO_3 particles. After being coated by PLL/Alg, microcapsules were obtained with the diameter of 2-4 μm. 10.0 μg of poly-PLL/Alg-PRV DNA microcapsules could cause rabbits' death by intramuscular injection. The identification of PCR shows that the death was caused by PRV infection. The results indicate that Poly-PLL/Alg microcapsules can mediate efficient transfection of DNA.