PREPARATION AND EVALUATION OF ZANAMIVIR-LOADED SOLID LIPID NANOPARTICLES

摘要

Zanamivir-loaded solid lipid nanoparticles (SLNs) were prepared by a double emulsion (W/O/W) solvent evaporation method, and the physicochemical properties of SLNs were evaluated. The SLNs prepared by optimal formulation had a spherical shape with a size of 183.9 nm, zeta potential of -53 mV, entrapment efficiency of 36.1% and loading efficiency of 0.32%. Zanamivir partially existed in the amorphous state in the freeze-dried nanoparticles, and carriers resulted in significant decrease in the intensity of the endothermic melting peak of the drug. In vitro release profiles were affected by the volume of the outer aqueous phase. The double (W/O/W) emulsion solvent evaporation method was effective for preparing zanamivir-loaded SLNs with relatively high entrapment efficiency.