Cytotoxicity and Biological Effects of Functional Nanomaterials Delivered to Various Cell Lines

摘要

Nanostructured materials have been found to be uptaken by various cell lines and highly affect their biological behavior. In this work, gold and silver metal nanoparticles as well as single wall carbon nanotubes were incubated separately and with apoptotic agents (Dexamethasone and Etoposide), in cell cultures of mouse long murine osteocytic bone cells (MLO-Y4 cells) and human cervical cancer cell line (Hela cells). The incubation of the nanomaterials with the cell cultures was carried out at two concentrations (0.5×10~(-9)mol/L and 10~(-12)mol/L) for 24 hours and the apoptotic agents (10~(-5)mol/L and 75×10~(-6)mol/L) were introduced for six hours. The cytotoxicity data revealed that the Au-NPs had a significant lower cytotoxic effect than the Ag-NPs and CNTs, values reflected by the percentage of the dead cells vs living cells and that the combination of the nanomaterials and the apoptotic agents had a combinatorial effect, resulting in a significantly larger number of cells that died. The results highlighted by this study could represent a major development for the delivery of specific drug molecules into cancer cells and tumors by nanomaterials.