Human glioma tumors detection by a portable visible resonance Raman analyzer with a hand-held optical fiber probe

摘要

Based on Visible Resonance Raman (VRR) method, we have developed a novel label-free portable VRR LRR2000?Raman analyzer with a portable fiber-optic probe and used it for the classification of human gliomas ex vivo and for theanalysis of changes in tumor chemical compositions in molecular level. The purpose of this study was to examine theperformance of the LRR2000 Raman analyzer as an optical biopsy tool for detecting human brain tumors compared to thecommercial laboratory HR800 and WITec300 micro confocal Raman spectroscopy instruments. As of 2018, a total 1,938VRR spectra were collected using LRR2000, HR800 and WITec300 Raman system, ex vivo. Identification of the fourgrades of glioma tumors and control tissues was performed based on the characteristic native molecular fingerprints.LRR2000 demonstrated consistent diagnostic results with HR800 and WITec300 Raman systems. LRR2000 showed theadvantages of high speed, convenience and low cost compared to the two confocal micro Raman systems. Using artificialintelligence (AI)-based analysis of part of the data, the cross-validated accuracy for identifying glioma tumors is ~90%compared with gold standard histopathology examination.