In this work we present the investigation of the interaction of fibronectin with different biomaterials. Since the amount,type and possible conformational changes of adsorbed proteins at the body-implant interface strongly influences theperformance of implants, this work is focusing on a more comprehensive understanding of these processes. To createvery stable and thin surface modification of a glass transducer with PEG, PU and PLA an immobilization strategy basedon silane chemistry was developed. To monitor the adsorption and desorption of fibronectin onto these biomaterialsurfaces, the reflectometric interference spectroscopy – a direct optical method – was applied. To estimate theassociation and dissociation rate constants, a biomolecular interaction analysis was performed within the kineticallylimited regime of the association process. Additionally, the influence of the pH value on the adsorption behavior isexemplary shown for the interaction of fibronectin on a glass surface. These experiments show high potential to givemore detailed insights into the complex processes during the implantation of foreign materials into the human body.
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