光反射干涉生物传感器对生物医用材料蛋白质吸附研究

摘要

应用东南大学生物医学工程系吴健雄实验室研制的RIfS生物传感器,对人工合成羟基磷灰石和H5050型聚氨酯两种材料对小牛血清白蛋白BSA、人血浆纤维蛋白原FIB和人免疫球蛋白IgG的吸附性能进行了实时原位动态研究.根据RIfS表征原理和蛋白质结构大小,引入了吸附层数计算公式,求出了2种实验材料对不同蛋白质的吸附层数.得出羟基磷灰石和H5050型聚氨酯材料对3种蛋白质实际吸附层数分别为:IgG1.0751,BSA0.9684,FIB0.7464和IgG0.8199,BSA0.7964,FIB0.6120.证实了用RIfS生物传感器进行生物材料生物相容性研究的可行性.%Using a RIfS set-up made by our laboratory we have investigatedthe kinetic behavior of bovine serum albumin (BSA), human fibrinogen (FIB) and human IgG adsorbed onto surfaces of hydroxyapatite and polyurethane H50-50. According to the principle of operation of RIfS and the molecular dimensions of three kinds of proteins we introduced a formula to calculate the adsorbed layers of proteins onto hydroxyapatite and polyurethane H50-50. The results show that the adsorbed layers of three kinds of proteins on the surface of hydroxyapatite are: IgG 1.075 1, BSA 0.968 4,FIB 0.746 4, and of polyurethane H50-50 are: IgG 0.819 9, BSA 0.796 4,FIB 0.612 0. The potential application of RIfS as a new analytical tool in the evaluation of the biocompatibility of biomaterials was confirmed.