Single cells diatom Chaetoceros muelleri investigated by homebuilt confocal fluorescence spectro-microscopy

摘要

Currently a combination of fluorescence microscopy with spectroscopic analysis in a balance way has played animportant role in providing new and exciting information for the in vivo study of cell, such as diatoms, photosyntheticmarine microorganisms. Diatoms contain photosynthetic pigments, carotenoid and chlorophylls molecules, in theirmachinery to harvest the sunlight energy for growth. Lacking of these pigments might influence to the overallmetabolisms of the cells. Here we reported our work to construct a simple homebuilt confocal fluorescence spectromicroscopyto study the cells of Chaetoceros muelleri diatom. The setup was design to get simultaneous data of theimage of the single fluorescence cell and the emission spectrum of it.