The problem that is posed byphase-only" objects, such as epithelial cells, for brightfield microscopy has resultedin development of several specialized imaging techniques including phase-contrast and DIC. In the past decadesthere has been increasing research on quantitative phase imaging (QPI), which enables real-time cellular dynamicsto be visualised and the 3-D morphology to be quantitatively measured. It has previously been demonstratedthat the DIC and phase-contrast images can be computationally generated using the phase-image provided byQPI. Recently, we have extended this approach to include Rheinberg. Although not as popular as phase contrastor DIC, Rheinberg illumination provides a form of label-free optical staining by introducing a multi-color filterinto the condenser plane of the microscope, enabling different features within the cell to be stained with differentcolors depending on their spatial-frequency content. We recently developed a theory for image formation withRheinberg illumination under the conditions of Kohler illumination from which an algorithm was developed thatcould simulate this process using the QPI image as input. In this paper we review and further develop thisapproach by testing it with multiple different modalities for recording the QPI image, namely digital holographicmicroscopy, which uses coherent illumination and spatial light interference microscopy, which makes use of whitelight. We examine a variety of samples including diatom and epithelial cells using a number of microscopeobjectives with different numerical apertures.
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