Method Development for Pesticide Residue Analysis in Farmland Soil using High Perfomance Liquid Chromatography

摘要

A method for the determination of diazinon and chlorantraniliprole in soil samples has been developed. The analyte was extracted with acetonitrile from farmland soil sample. Determination and quantification of diazinon and chlorantraniliprole were perfomed by high perfomance liquid chromatography (HPLC) with an UV detector. Several parameters of HPLC method were optimized with respect to sensitivity, high resolution of separation, and accurate determination of diazinon and chlorantraniliprole. Optimum conditions for the separation of two pesticides were eluent composition of acetonitrile: water ratio of 60:40,0.4 mL/min of flow rate, and 220 nm of wavelength. Under the optimum conditions, diazinon linearity was in the range from 1-25 ppm with R~2 of 0.9976, 1.19 mgL~(-1)LOD, and 3,98 mgL~(-1) LOQ; while the linearity of chlorantraniliprole was in the range from 0.2-5 mgL~(-1) with R~2 of 0.9972, 0.39 mgL~(-1) LOD, and 1.29 mgL~(-1) LOQ. When the method was applied to the soil sample, both pesticides showed acceptable recoveries for real sample of more than 85%: thus, the developed method meets the validation requirement. Under this developed method, the concentrations of both pesticides in the soil samples were below the LOD and LOQ (0.577 mgL~(-1) for diazinon and 0.007 mgL~(-1) for chlorantraniliprole). Therefore, it can be concluded that the soil samples used in this study have neither diazinon nor chlorantraniliprole.