Preparation and properties of magnetic-fluorescent endoglin aptamer nanoprobe

摘要

In this study, a kind of magnetic-fluorescent endoglin aptamer nanoprobe, Cy5.5-End-Fe3O4/KCTS, was synthesized with magnetic functions of Fe3O4/KCTS nanoparticles and the fluorescence ability of Cy5.5 through SMCC cross-linking with endoglin aptamer labeled with Cy5.5. The microstructure, fluorescence and magnetic properties of the samples were investigated and discussed by Fourier transform-infrared (FT-IR) spectra, X-ray diffraction (XRD), transmission electron microscopy (TEM), photoluminescence spectra, dynamic light scattering (DLS) and Zeta potential. The magnetic-fluorescent aptamer nanoprobe has an average particle size of about 240 nm, Zeta potential of -8.9 mV, excellent fluorescence and magnetic properties. This study provides a new MRI/fluorescent bimodal molecular probe for early diagnosis of hepatocellular carcinoma.