Studies of the bovine milk microbiome have reported the presence of DNA sequences from previously uncultured bacteria in the healthy mammary gland but have used different methods of sample collection (Oikonomou et al., 2012; Kuehn et al., 2013). Thesestudies have not reported the sampled cows' bedding type even though cows housed on different bedding types are exposed to different bacterial species (Rowbotham and Ruegg, 2016). The objective of this study was to determine if the milk microbiota was associated with method of sampling and bedding type.
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