Optimization of Serratia nematodiphila using Response surface methodology to silver nanoparticles synthesis for aquatic pathogen control

摘要

In this study, we used bacterial strain Serratia nematodiphila for the synthesis of silver nanoparticles using optimized biomass growth. In this RSM study the variables such as sodium sulphate (g / L) (0.5, 1, 1.5), magnesium sulphate (g /L) (0.3, 0.5, 0.7), pH (6.4, 7.4, 8.4.), temperature (25, 30, 35 C) and Sodium lactate, Peptone have been used for the maximum production of biomass. We got very good a result for the silver nanoparticles was confirmed using UV-vis spectrophotometer and transmission electron microscope. Finally, we concluded that the using of RSM for nanoparticles synthesis may use in industrial biotechnology and related technologies for large scale production.