Biodegradation of Diesel Oil Hydrocarbons Using Bacillus subtilis InaCC B289 and Pseudomonas aeruginosa InaCC B290 in Single and Mixed Cultures

摘要

Biodegradation is a method used to control diesel oil pollution, by utilizing bacteria to degrade the hydrocarbon compound. Bacillus subtilis and Pseudomonas aeruginosa are known to be able to degrade diesel oil. The objective of this study was to investigate the ability of both single and mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCC B290 to degrade hydrocarbon compounds. Parameters were measured for 25 days and included pH, optical density (OD) and dissolved oxygen (DO). The results revealed that pH, OD, and DO were not significantly different (p> 0.05). Furthermore, the percentage of degradation was analyzed using gas chromatography-mass spectrometry (GC-MS) and showed that the degradation of the hydrocarbon compounds found in diesel oil was higher with mixed cultures of B. subtilis InaCC B289 and P. aeruginosa InaCCB290 (57.56%), compared to single cultures of B. subtilis InaCCB289 (37.53%) and P. aeruginosa InaCC B290 (36.50%).