Study on Apoptosis of Human Hepatoma Cell line HepG-2 Induced by Total Alkaloids in H.littoralis

摘要

The purpose of this study is to investigate the effect of Total Alkaloids in H.littoralis on the induction of apoptosis involved in Human Hepatoma Cell line HepG-2. HepG-2 cells were treated with different concentrations of Alkaloids in H.littoralis at different time. MTT method was used to study Alkaloids in H.littoralis anti-tumor activity. Morphology observation was performed to determine the effects of Alkaloids in H.littoralis on apoptosis of HepG-2 cells. Flow cytometry (FCM) was used to detect the cell apoptosis rate and analyze the protein expression of Fas. The results showed Alkaloids in H.littoralis could inhibit the growth of HepG-2 cells, and the apoptosis morphology for 72h treatment was obvious. FCM indicated that the percentage of apoptotic cells increased with the raised concentration and the protein expression of Fas was increased after treated by Alkaloids in H.littoralis for 72h. In conclusion, Alkaloids of H.littoralis could induce HepG-2 cells apoptosis, and the mechanism may be related to death receptor pathway.