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Single-Shot Phase Shifting Interferometry for Microscopic Measurements of Non-Birefringent Transmissive Phase Samples

机译:用于非双折射透射相样品的微观测量的单次拍摄相移干涉测量

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Its well known that there is a variety of techniques to obtain n-phase shifts in one shot, and most of them use diffractive elements, holographic ones or pixelated phase masks attached to a CCD camera, among others, to generate from 4 to 9 interferograms simultaneously; nevertheless, some of the components utilized in this arrangements are still expensive, and in order to reduce the cost of operations, we developed an alternative system that does not use diffractive elements, in this research we present a simultaneous phase-shifting interferometer based on polarizing coupled interferometers, this device can be measured the optical phase of non-birefringent microscopic phase samples, generating four interferograms in single capture of the ccd camera with relative phase shifts of π/2. In order to present the capabilities of the system, the results obtained for the phase measurement of Red Blood Cells (RBC) and non-birefringent transparent samples are presented.
机译:其众所周知,有多种技术在一次拍摄中获得N相移位,并且大多数使用衍射元件,全息元或像素化相位掩模附着在CCD相机等中,以产生4到9个干涉图。同时;然而,在该布置中使用的一些组件仍然是昂贵的,并且为了降低操作成本,我们开发了一种不使用衍射元件的替代系统,在本研究中,我们介绍了一种基于偏振的同时移相的干涉仪耦合干涉仪,该装置可以测量非双折射微观相位样本的光学相位,在单一捕获中产生四个干涉图,其中CCD相机具有相对相位偏移的π/ 2。为了呈现该系统的能力,提出了对红细胞(RBC)和非双折射透明样品的相位测量获得的结果。

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