Formulation and evaluation of serum from red, brown and green algae extract for anti-aging base material

摘要

Red, brown and Green algae contain antioxidant and have the potential as anti-aging but still rarely developed for cosmetic ingredients. The purpose of this study was to investigate the chemical constituent, total phenol and flavonoid content, antioxidant activity and toxicity of algae extract and formulated into serum preparation. Padina Australis, Ulva Reticulata, Sargassum Oligocystum, dan Euchema Cottonii contain chemical constituent hexadecanoic acid, methyl ester, fatty acid and phytol which possess antioxidant activity. P. australis has the highest total phenol and flavonoid content that is 106 mgGAE/100 gr and 30.516 mgQE/g respectively. Antioxidant activity of P. australis, U. reticulata, E. cottoni and S. oligocystum is stated in IC_(50) value which are 2693.33, 2679.16, 4407.57 and 1700 μg/ml respectively wherein it still considered as low antioxidant activity. Algae extract in this study also proven to have low toxicity with LC_(50) higher than 1000 ppm. Algae crude extract was formulated into anti-aging serum formulation and evaluated for one month by accelerated stability test method. This stability test observed physical properties pH, viscosity, refractive index, and specific gravity and chemical properties. Based on the stability test results on each anti-aging serum formulas, formula with E. cottoni extract showed the stable formulation for serum preparation with no significant chemical and physical change due to temperature alteration during accelerated stability test.