Immobilization of glucoamylase on stimuli-sensitive macroporous semi-interpenetrating stimuli-sensitive hydrogel carriers

摘要

Based on the special phase transition property of copolymer hydrogel of N-is opropylacrylamide, the immobilization of glucoamylase on five macro porous semi-interpenetrating stimuli-sensitive hydrogel carriers was studied. Four good preparations were screened, i.e. GelNIPAm/PAEMA-2.5, Gel NAEP/PAEMA-2.5, Gel NAP/PAEMA-2.5 and Gel NDEP/PAEMA-2.5, the immobilization yield °C activity) of them were 11.4%, 12.7%, 14.9% and 15.3% respectively under optimal conditions. The most preferred temperature of these immobilized glucoamylase was 30°C, and the adapted pH was about pH 4.5. The activities of the latter three photo-sensitive preparations could be adjusted by UV irradiation during their phase transition. After month storage, the activity retention rate was no less than 80% for every one preparations cross-linked with 2.5 vol% of glutaraldehyde.