Cloning and Expression of cry9Ea10 Gene from Bacillus Thuringiensis Strain GZ2 Isolated from Infected Hyphantria Cunea Larvae

摘要

A novel Bacillus thuringiensis (Bt) strain GZ2, was isolated from infected Hyphantria cunealarvae. The bioassay results showed that GZ2 had insecticidal activity to H.cunea larvae. The genotypes of GZ2 were identified by PCR-RFLP method. GZ2 was identified to harbor cry9Ea10 gene and the full length of cry9Ea10 was cloned and sequenced. The engineering strain of Bt carrying cry9Ea10gene wasconstructed successfully. SDS-PAGE analysis confirmed that the expression of cry9Ea10 as ～130kDa protein. And Cry9Ea10 protein produced by Bt engineeringstra inexhibited a high toxicity to H.cunealarvae.