Injury Difference of the Un-Aggregated and Aggregated Calcium Oxalate Monohydrate on Human Kidney Proximal Tubular Epithelial Cells

摘要

Purpose: To study the injury difference of the un-aggregated and aggregated calcium oxalate monohydrate (COM) on human kidney proximal tubular epithelial (HKC) cells, and to discuss the molecular and cellular mechanism of kidney stone formation. Methods: The cell injure by crystals was evaluated by measuring the cell viability, malonaldehyde (MDA) release, and the expression level of osteopontin (OPN). Scanning electron microscope (SEM) was used to observe the HKC-COM crystal adhesion. Results: After adhesion to COM, cell vitality dropped, both MDA release and OPN expression increased. The crystal injury in HKC was in a time-dependent manner within 24 h. aggregated COM caused more serious injury than un-aggregated COM, and the adhesive capacity of aggregated COM was also obviously stronger than un-aggregated COM. Conclusions: Aggregated COM crystals enhanced the risk of calcium oxalate stone formation much stronger than un-aggregated COM crystals.